worthington 腦組織分離指南

原代細胞分離中,針對腦組織涉及到微血管內皮、腦內皮細胞、神經元和小膠質細胞、微血管等等的解離通常會使用酶,如何選擇合適的酶來完成對應腦組織的解離,以及原代細胞采集的常規操作,?艾美捷針對腦相關的組織分類解離分種屬、不同的細胞、酶組合推薦,推薦濃度、培養基以及參考文獻,詳細匯總指南:

腦組織分離指南

 

本技術指南描述了標準實驗室程序; 為建立細胞分離方案提供了一種合乎邏輯的實驗方法; 并列出了許多組織特異性參考資料,以幫助開發有效的方法。更多其他組織的解離指南,點擊查看:各組織分離指南。

種屬 種屬詳情 細胞名稱 酶組合推薦,含推薦濃度 培養基 參考文獻
Bovine 微血管內皮 / Microvascular endothelial Neutral Protease: 0.005% Medium 199 Kanda, T., Yoshino, H., Ariga, T., Yamawaki, M., and Yu, R.:?Glycosphingolipid Antigens in Cultured Microvasular Bovine Brain Endothelial Cells: Sulfoglucuronosyl Paragloboside as a Target of Monoclonal IgM in Demyelinative Neuropathy,?J Cell Biol?126 (1), 235, 1994
Bovine 腦內皮細胞 / Brain endothelial cells Collagenase Type 2: 0.35% DMEM Wolburg H, Neuhaus J, Kniesel U, Krauss B, Schmid EM, Ocalan M, Farrell C, Risau W:?Modulation of tight junction structure in blood-brain barrier endothelial cells. Effects of tissue culture, second messengers and cocultured astrocytes,?J Cell Sci?107, 1347, 1994
Bovine 內皮 / Endothelial Neutral Protease: 0.125% MEM Miller, D., Audus, K., and Borchardt, R.: Application of Cultured Endothelial Cells of the Brain Microvasculature in the Study of the Blood-Brain Barrier,?J Tiss Cul Meth 14, 217, 1992
Bovine, adult 腦動脈,內皮 / Cerebral artery,Endothelial Collagenase: 0.2% HBSS Machi, T., Kassell, N.F., and Scheld, W.M.:?Isolation and Characterization of Endothelial Cells From Bovine Cerebral Arteries,?In Vitro Cell Dev Biol 26, 291, 1990
Bovine 毛細血管內皮 / Capillary endothelial Collagenase: 0.1% DMEM Estrada, C., Bready, J., Berliner, J., and Cancilla, P.:?Choline Uptake by Cerebral Capillary Endothelial Cells in Culture,?J Neurochem 54, 1467, 1990
Bovine 內皮 / Endothelial Neutral Protease: 0.5% MEM Audus, K., and Borchardt, R.: Characterization of an?In Vitro?Blood-Brain Barrier Model System for Studying Drug Transport and Metabolism,?Pharm Res 3 (2), 81, 1986
Bovine 內皮,腦動脈 / Endothelial,Brain arteries Collagenase Type 2: 0.2% Dulbecco's PBS Goetz, I.., Warren, J., Estrada, C., Roberts, E., and Krause, D.:?Long-Term Cultivation of Arterial and Capillary Endothelium From Adult Bovine Brain,?In Vitro Cell Dev Biol 21, 172, 1985
Calf 少突膠質細胞,神經 / Oligodendroglia,Neural Trypsin: 0.1% (see reference) Poduslo, S., Miller, K., and McKhann, G.:?Metabolic Properties of Maintained Oligodendroglia Purified from Brain,?J Biol Chem 253, 1592, 1978
豚鼠 Guinea-pig, 200-400 g 神經元 / Neurons Trypsin: 0.06-0.08% PIPES saline Kay, A.R., and Wong, R.K.S.:?Isolation of Neurons Suitable for Patch-Clamping from Adult Mammalian Central Nervous Systems,?J Neurosci Methods 16, 227, 1986
Human 小膠質細胞 / Microglia Collagenase Type 1: 300 u/ml;
Trypsin: 0.125%
DMEM Mizee, M., Miedema, S., van der Poel, M., Adelia , S., van Strien, M., Melief, J., Smolders, J., Hendrickx, D., Heutinck, K., Hamann, J. and Huitinga, I.:?Isolation of Primary Microglia from the Human Post-Mortem Brain: Effects of Ante- and Post-Mortem Variables.,?Acta Neuropathol?5, 16, 2017
Human, adult 神經元 / Neuronal Papain: 20 u/ml Neurobasal Spaethling, J., Na, Y., Lee, J., Ulyanova, A., Baltuch, G., Bell, T., Brem, S., Chen, H., Dueck, H., Fisher, S., Garcia, M., Khaladkar, M., Kung, D., Lucas, T., O'Rourke, D. and Stefanik, D.:?Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics.,?Cell Rep?18, 791-803, 2017
Human, fetal and mature 星形膠質細胞和神經元 / Astrocytes and neurons Papain: 7.5-20 u/ml RPMI Zhang, Y., Sloan, S., Clarke, L., Caneda, C., Plaza, C., Blumenthal, P., Vogel, H., Steinberg, G., Edwards, M., Li, G., Duncan, J., Cheshier, S., Shuer, L., Chang, E., Grant, G., Gephart, M. and Barres, B.:?Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse.,?Neuron 89, 37-53, 2016
Human 小膠質細胞 / Microglia Neutral Protease: 10 u/ml;
Papain: 2.5 u/ml
Hibernate A Rustenhoven, J., Park Thomas, I., Schweder, P., Scotter, J., Correia, J., Smith, A., Gibbons, H., Oldfield, R., Bergin, P., Mee, E., Faull, R., Curtis, M., Scott, G. and Dragunow, M.:?Isolation of Highly Enriched Primary Human Microglia for Functional Studies.,?Sci Rep?6, 19371, 2016
Human, 25-81 yr 腦腫瘤 / Brain tumor Neutral Protease: 0.11 u/ml;

Collagenase Type 4: 0.05%;

Hyaluronidase: 1,000 u/ml;

Deoxyribonuclease I: 5 u/ml;

HBSS Volovitz, I, Shapira, N., Ezer, H., Gafni, A., Lustgarten, M., Alter, T., Ben-Horin, I., Barzilai, O., Shahar, T., Kanner, A., Fried, I., Veshchev, I., Grossman, R. and Ram, Z,:?A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells.,?BMC Neurosci?17, 30, 2016
Human 淀粉樣蛋白原纖維 / Amyloid Fibrils Collagenase Type 3: 0.02%;
Deoxyribonuclease I: 0.001%
50mM Tris Scherpelz, K., Lu, J.,Tycko, R. and Meredith, S.:?Preparation of Amyloid Fibrils Seeded from Brain and Meninges,?Methods Mol Biol 1345, 299, 2016
腦腫瘤 / Brain tumor Collagenase Type 1: 0.04%;

Hyaluronidase: 0.01%;

Deoxyribonuclease I: 0.02%;

Neutral Protease: 0.008%;

DMEM/F12 Hussein, D., Punjaruk, W., Storer, L., Shaw, L., Othman, R., Ottoman, R., Peet, A., Miller, S., Bandopadhyay, G., Heath, R., Kumari, R., Bowman, K., Braker, P., Rahman, R., Jones, G., Watson, S. and Lowe, J.:?Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion.,?Neuro Oncol?13, 70-83, 2011
Human 腫瘤 / Tumor Collagenase Type 4: 0.1%;
Hyaluronidase: 0.07%;
Deoxyribonuclease I: 0.04%
see reference Sauvageot, C., Weatherbee, J., Kesari, S., Winters, S., Barnes, J., Dellagatta, J., Ramakrishna, N., Stiles, C., Kung, A., Kieran, M. and Wen, P.:?Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells.,Neuro Oncol Vol. 11,, , 109, 2009
Human 小膠質細胞 / Microglia Trypsin: 0.25%;
Deoxyribonuclease I: .005%
DMEM/F12 Klegeris Andis, McGeer PatrickL:?Chymotrypsin-like proteases contribute to human monocytic THP-1 cell as well as human microglial neurotoxicity,?Glia 51, 56-64, 2005
Human 微血管 / Microvessels Collagenase Type 4: 0.1% DMEM Gerhart, D. Z., Broderius, M. A., and Drewes, L. R.:?Cutlured Human and Canine Endothelial Cells from Brain Microvessels,?Brain Res Bull 21, 785, 1988
Human 神經元 / Neuronal Deoxyribonuclease I: 10 痢/ml Tris-HCl, 50 mM, CaCl2, 2 mM Roher, A.E., Palmer, K.C., Chau, V., and Ball, M.J.:?Isolation and Chemical Characterization of Alzheimer's Disease Paired Helical Filament Cytoskeletons:Differentiation from Amyloid Plaque Core Protein,?J Cell Biol?107, 2703, 1988
Human, 15-54 years 微血管 / Microvessels Collagenase: 0.1% Serum-free modified Lewis medium Vinters, H.V., Reave, S., Costello, P., Girvin, J.P., and Moore, S.A.:?Isolation and Culture of Cells Derived From Human Cerebral Microvessels,?Cell Tissue Res 249, 657, 1987
昆蟲 Drosophilia 神經元 / Neurons Papain: 50 u/ml Modified Dissecting Saline Hadzic, T., Park, D., Abruzzi, K., Yang, L., Trigg, J., Rohs, R., Rosbash, M. and Taghert, P.:?Genome-Wide Features of Neuroendocrine Regulation in Drosophila by the Basic Helix-Loop-Helix Transcription Factor DIMMED.,?Nucleic Acids Res?43, 2199-215, 2015
昆蟲 Drosophila 神經元 / Neurons Papain: 20 u/ml Saline Gu, H. and O'Dowd, D.:?Whole Cell Recordings from Brain of Adult Drosophila.,?J Vis Exp?6, 248, 2007
昆蟲 Drosophila 神經元 / Neurons Papain: 4 u/ml DMEM Sicaeros, B. Campusano, J. and O'Dowd, D.:?Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae.,?J Vis Exp?4, 200, 2007
昆蟲 Drosophilia 肯揚 / Kenyon Papain: 20 u/ml Recording saline Gu, H. and O'Dowd, D.:?Cholinergic Synaptic Transmission in Adult Drosophila Kenyon Cells in Situ.,?J Neurosci 26, 265-72, 2006
Monkey, rhesus 腦細胞 / Brain cells Collagenase Type 2: 500 u/ml;
Deoxyribonuclease I: 28 u/ml
HBSS Marcondes MC, Burudi EM, Huitron-Resendiz S, Sanchez-Alavez M, Watry D, Zandonatti M, Henriksen SJ, Fox HS:?Highly activated CD8(+) T cells in the brain correlate with early central nervous system dysfunction in simian immunodeficiency virus infection,?J Immunol 167, 5429-38, 2001
小鼠 Adult and neonatal 小膠質細胞,星形膠質細胞,淋巴細胞 / Microglia, astrocytes, lymphocytes Collagenase Type 2: 120 u/ml;
Papain: 20 u/ml;
Neutral Protease: 1.2 u/ml;
Deoxyribonuclease I: 20 u/ml
EBSS Calvo, Belen, Rubio, F., Fernandez, M. and Tranque, P.:?Dissociation of Neonatal and Adult Mice Brain for Simultaneous Analysis of Microglia, Astrocytes and Infiltrating Lymphocytes by Flow Cytometry.,IBRO Rep Vol. 8,, , 36-47, 2020
小鼠 Mouse, adult 星形膠質細胞,小膠質細胞,內皮細胞,少突膠質細胞 / Astrocytes, microglia, endothelia, oligodendrocytes Papain: 20 u/ml;
Collagenase Type 1: 0.01%
HBSS Swartzlander, D., Propson, N., Roy, E., Saito, T., Saido, T., Wang, B. and Zheng, H.:?Concurrent Cell Type-Specific Isolation and Profiling of Mouse Brains in Inflammation and Alzheimer's Disease.,?JCI Insight?3, , 2018
小鼠 Mouse 神經元 / Neurons Papain: 10 u/ml;
Deoxyribonuclease I: 10 u/ml
Artificial CSF Kramer, D., Risso, D., Kosillo, ., Ngai, J. and Bateup, H.:?Combinatorial Expression of Grp and Neurod6 Defines Dopamine Neuron Populations with Distinct Projection Patterns and Disease Vulnerability,eNeuro Vol. 5,, , 1, 2018
小鼠 Mouse, fetal and mature 星形膠質細胞和神經元 / Astrocytes and neurons Papain: 7.5-20 u/ml RPMI Zhang, Y., Sloan, S., Clarke, L., Caneda, C., Plaza, C., Blumenthal, P., Vogel, H., Steinberg, G., Edwards, M., Li, G., Duncan, J., Cheshier, S., Shuer, L., Chang, E., Grant, G., Gephart, M. and Barres, B.:?Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse.,?Neuron 89, 37-53, 2016
小鼠 Mouse 脈絡叢 / Chloroid plexus Collagenase Type 4: 400 u/ml PBS Baruch, K., Rosenzweig, N., Kertser, A., Deczkowska, A., Sharif, A., Spinrad, A., Tsitsou-Kampeli, A., Sarel, A., Cahalon, L. and Schwartz, M.:?Breaking Immune Tolerance by Targeting Foxp3(+) Regulatory T Cells Mitigates Alzheimer's Disease Pathology.,?Nat Commun?6, 7967, 2015
小鼠 Mouse, 3 week 神經 / Neural Papain: 20 u/ml PBS Luo,Y.,Coskun, V., Liang, A.,Yu, J., Cheng, L.,Ge, W., Shi, Z., Zhang, K., Li, C., Lin, H., Luo, D., Wang, J., Lin, C., Dai, Z., Zhu, H., Zhang, J., Liu, J., Liu, H.,deVellis, J., Horvath, S., Sun, Y. and Li, S.:?Different Patterns of Molecular Forms of Somatostatin are Released by the Rat Median Eminence and Hypothalamus.,?Cell?161, 1175, 2015
小鼠 Mouse 腦隔離白細胞 / Brain-sequestered leukocytes Collagenase Type 4: 0.05%;
Deoxyribonuclease I: 2 u/ml
RPMI Ryg-Cornejo, V., Ioannidis, L. and Hansen, D.:?Isolation and Analysis of Brain-Sequestered Leukocytes from Plasmodium Berghei ANKA-Infected Mice.,?J Vis Exp?71, e50112, 2013
小鼠 Mouse 腦周細胞 / Cerebral pericytes Collagenase Type 2: 0.1%;
Deoxyribonuclease I: 30 u/ml
DMEM Shah. G., Price, T., Banks, W., Morofuji, Y., Kovac, A., Ercal, N., Sorenson, C., Shin, E., Sheibani, N.:?Pharmacological Inhibition of Mitochondrial Carbonic Anhydrases Protects Mouse Cerebral Pericytes from High Glucose-Induced Oxidative Stress and Apoptosis.,?J Pharmacol Exp Ther 344, 637-45, 2013
小鼠 Mouse, P13-15 膠質細胞 / Glial Papain: 20 u/ml;
Deoxyribonuclease I: 0.0005%
EBSS Haseleu, J., Anlauf, E., Blaess, S., Endl, E. and Derouiche, A.:?Studying Subcellular Detail in Fixed Astrocytes: Dissociation of Morphologically Intact Glial Cells (DIMIGs).,?Front Cell Neurosci?7, 54, 2013
小鼠 Mouse, P0-P15 星形膠質細胞 / Astrocytes Papain: 5 u/ml;
Deoxyribonuclease I: 0.004%
DPBS Foo, L.:?Purification of Rat and Mouse Astrocytes by Immunopanning.,?Cold Spring Harb. Protoc. 2013, 421-32, 2013
小鼠 Mouse, adult 神經元 / Neurons PDS kit: with modifications DMEM/F12 Saxena, A., Wagatsuma, A., Noro, Y., Kuji, T., Asaka-Oba, A., Watahiki, A., Gurnot, C., Fagiolini, M., Hensch, T. and Carninci, P.:?Trehalose-Enhanced Isolation of Neuronal Sub-Types from Adult Mouse Brain.,?Biotechniques?52, 381, 2012
小鼠 Mouse, 1-2 day 少突膠質細胞,背根神經節 / Oligodendrocytes, dorsal root ganglia Papain: 0.15%;
Deoxyribonuclease I: 0.006%
DMEM O'Meara, R., Ryan, S., Colognato, H. and Kothary, R.:?Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-Cultures from Post-Natal Murine Tissues.,?J Vis Exp?54, 3324, 2011
小鼠 Mouse, 6-12 week 血管平滑肌細胞 / Vascular smooth muscle cells Papain: 0.05%;
Collagenase Type 4: 0.15%;
Elastase: 0.05%
PBS Chung, W., Farley, J., Swenson, A., Barnard, J., Hamilton, G., Chiposi, R. and Drummond, H.:?Extracellular Acidosis Activates ASIC-like Channels in Freshly Isolated Cerebral Artery Smooth Muscle Cells.,?Am J Physiol Cell Physiol?298, C1198, 2010
小鼠 Mouse, 4-6 day 神經元 / Neurons PDS kit: with modifications HBSS Lee, H., Greene, L., Mason, C. and Manzini, M.:?Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons.,?J Vis Exp?23, 990, 2009
小鼠 Mouse, postnatal 星形膠質細胞 / Astrocytes Trypsin: 0.25%;
Deoxyribonuclease I: 1,000 u/ml
HBSS Sher, F., Rossler, R., Brouwer, N., Balasubramaniyan, V., Boddeke, E. and Copray, S.:?Differentiation of Neural Stem Cells Into Oligodendrocytes: Involvement of the Polycomb Group Protein Ezh2.,?Stem Cells 26, 2875, 2008
小鼠 Mouse, neonatal 神經元 / Neurons Papain: 20 u/ml Neurobasal Fasano, C., Thibault, D. and Trudeau, L.:?Culture of Postnatal Mesencephalic Dopamine Neurons on an Astrocyte Monolayer,Current Protocols in Neuroscience Vol. 44,, , 3.21.1, 2008
小鼠 Mouse, embryonic and postnatal 皮層神經元 / Cortical neurons Papain: 4-10 u/ml Neurobasal Hilgenberg, L. and Smith, M.:?Preparation of Dissociated Mouse Cortical Neuron Cultures.,?J Vis Exp?10, 562, 2007
小鼠 Mouse 神經球 / Neurosperes PDS kit: per instructions DMEM/F12 Klein C, Butt SJ, Machold RP, Johnson JE, and Fishell G.:?Cerebellum- and forebrain-derived stem cells possess intrinsic regional character,?Development?132, 4497, 2005
小鼠 Mouse, 1 day 神經祖細胞 / Neural progenitor PDS kit: per instructions see reference Seaberg, R., Smukler, S. and Van der Kooy, D.:?Intrinsic Differences Distinguish Transiently Neurogenic Progenitors from Neural Stem Cells in the Early Postnatal Brain.,?Dev Biol 278, 71, 2005
小鼠 Mouse 顆粒細胞前體,前腫瘤細胞和腫瘤細胞 / Granule cell precursors, pre-neoplastic and tumor cells Papain: 10 u/ml;
Deoxyribonuclease I: 250 u/ml
Neurobasal/B27 Oliver, T., Read, T., Kessler, J., Mehmeti, A., Wells, J., Huynh, T., Lin, S. and Wechsler-Reya, R.:?Loss of Patched and Disruption of Granule Cell Development in a Pre-Neoplastic Stage of Medulloblastoma.,?Development?132, 2425, 2005
小鼠 Mouse 皮層神經元 / Cortical neurons PDS kit: per instructions Neurobasal Hernandez, F., Perez, M., Lucas, J., Mata, A., Bhat, R. and Avila, J.:?Glycogen Synthase Kinase-3 plays a Crucial Role in Tau Exon 10 Splicing and Intranuclear Distribution of SC35. Implications for Alzheimer's Disease.,?J Biol Chem 279, 3801, 2004
小鼠 Mouse, 3 day 小膠質細胞 / Microglia Papain: 90 u/ml;
Deoxyribonuclease I: 2000 u/ml
Eagle's MEM Nishioku T, Hashimoto K, Yamashita K, Liou SY, Kagamiishi Y, Maegawa H, Katsube N, Peters C, von Figura K, Saftig P, Katunuma N, Yamamoto K, Nakanishi H:?Involvement of cathepsin E in exogenous antigen processing in primary cultured murine microglia,?J Biol Chem 277, 4816, 2002
小鼠 Mouse, SD, 8-12 week 小膠質細胞 / Microglia Trypsin: 0.125%;
Collagenase Type 2: 0.01%;
Deoxyribonuclease I: .005%
RPMI-1640 O'Donnell SL, Frederick TJ, Krady JK, Vannucci SJ, Wood TL:?IGF-I and microglia/macrophage proliferation in the ischemic mouse brain,?Glia 39, 85, 2002
小鼠 Mouse, 2-5 day 后黑質 / Postnal substantia nigra PDS kit: per instructions see reference Smeyne Michelle, Smeyne RichardJ:?Method for culturing postnatal substantia nigra as an in vitro model of experimental Parkinson's disease,?Brain Res Brain Res Protoc 9, 105-11, 2002
小鼠 Mouse, embryonic 皮質祖細胞 / Cortical progenitors PDS kit: per instructions Serum free medium Estivill-Torrus, G., Pearson, H., Van Heyningen, V., Price, D. and Rashbass, P.:?Pax6 is Required to Regulate the Cell Cycle and the Rate of Progression from Symmetrical to Asymmetrical Division in Mammalian Cortical Progenitors.,?Development?129, 455, 2002
小鼠 Mouse, 1 day 神經元和神經膠質 / Neurons and glia PDS kit: per instructions Neurobasal Martin-Aparicio, E., Yamamoto, A., Hernandez, F., Hen, R., Avila, J. and Lucas, J.:?Proteasomal-Dependent Aggregate Reversal and Absence of Cell Death in a Conditional Mouse Model of Huntington's Disease.,?J Neurosci 21, 8772, 2001
小鼠 Mouse 小腦顆粒細胞前體 / Cerebellar granule cell precursors Papain: 0.435%;
Deoxyribonuclease I: 0.05%
EBSS Miyazawa K, Himi T, Garcia V, Yamagishi H, Sato S, and Ishizaki Y.:?A role for p27/Kip1 in the control of cerebellar granule cell precursor proliferation,?J Neurosci 20, 5756, 2000
小鼠 Mouse 星形膠質細胞 / Astrocytes Trypsin: 0.25% DMEM Lim, D, and Alvarez-Buylla, A.:?Interaction Between Astrocytes and Adult Subventricular Zone Precursors Stimulates Neurogenesis,?Proc Natl Acad Sci U S A 96, 7526, 1999
小鼠 Mouse, newborn 海馬細胞 / Hippocampal cells Papain: 10 u/ml DMKM Jun K, Choi G, Yang SG, Choi KY, Kim H, Chan GC, Storm DR, Albert C, Mayr GW, Lee CJ, Shin HS:?Enhanced hippocampal CA1 LTP but normal spatial learning in inositol 1,4,5-trisphosphate 3-kinase(A)-deficient mice,?Learn Mem?5, 317-30, 1998
小鼠 Mouse, SWR or CF1, 1-3 months 乳頭,味覺感受器 / Papillae, taste receptor Pronase E: 0.15% Carbonate-Phosphate buffer Spielman, A., Mody, I., Brand, J., Whitney, G., MacDonald, J., and Salter, M.:?A Method for Isolating and Patch-Clamping Single Mammalian Taste Receptor Cells,?Brain Res 503, 326, 1989
小鼠 Mouse, 0-30 day 神經 / Neural Trypsin NF 1:250: 0.25% BSS Shrier, B., Wilson, S., and Nirenberg, M.:?Cultured Cell Systems and Methods for Neurobiology,?Vol. 32,, , 765, 1974
綿羊 Ovine 神經元 / Neurons Papain: 0.2%;
Deoxyribonuclease I: 0.1%
Hibernate Reddy, R., Amodei, R., Estill, C., Stormshak, F., Meaker, M. and Roselli, C.:?Effect of Testosterone on Neuronal Morphology and Neuritic Growth of Fetal Lamb Hypothalamus-Preoptic Area and Cerebral Cortex in Primary Culture.,?PLoS ONE?10, e0129521, 2015
綿羊 Ovine 神經元 / Neurons PDS kit: per instructions DMEM Lepore G, Gadau S, Mura A, Zedda M, Farina V:?Aromatase Immunoreactivity in Fetal Ovine Neuronal Cell Cultures Exposed to Oxidative Injury.,?Eur J Histochem 53, e28, 2009
綿羊 Lamb 少突膠質細胞,神經 / Oligodendroglia,Neural Trypsin: 0.1% (see reference) Poduslo, S., Miller, K., and McKhann, G.:?Metabolic Properties of Maintained Oligodendroglia Purified from Brain,?J Biol Chem 253, 1592, 1978
Mini pigs, Yucatan (Susscrofa Yucatan), 4-6 months 微血管 / Microvascular Collagenase: 0.1% HBSS Robinson, D.H., Kang,Y., Deschner, S.H., and Nielsen, T.B.:?Morphologic Plasticity and Periodicity : Porcine Cerebral Microvascular Cells in Culture,?In Vitro Cell Dev Biol 26, 169, 1990
大鼠 Rat, SD, 1-2 day 少突膠質前體細胞 / Oligodendrocyte precursor cells Papain: 16.5 u/ml HBSS Kurachi, M., Mikuni, M. and Ishizaki, Y.:?Extracellular Vesicles from Vascular Endothelial Cells Promote Survival, Proliferation and Motility of Oligodendrocyte Precursor Cells.,?PLoS ONE?11, e0159158, 2016
大鼠 Rat, SD, E18 神經元 / Neurons Papain: 20 u/ml HBSS Folts, C., Scott-Hewitt, N., Proschel, C., Mayer-Proschel, M. and Noble, M.:?Lysosomal Re-acidification Prevents Lysosphingolipid-Induced Lysosomal Impairment and Cellular Toxicity.,?PLoS Biol?14, e1002583, 2016
大鼠 Rat, P0-P15 星形膠質細胞 / Astrocytes Papain: 5 u/ml;
Deoxyribonuclease I: 0.004%
DPBS Foo, L.:?Purification of Rat and Mouse Astrocytes by Immunopanning.,?Cold Spring Harb. Protoc. 2013, 421-32, 2013
大鼠 Rat, SD, neonatal 神經元 / Neurons Papain: 20 u/ml Neuro medium Kaiser, O., Aliuos, P., Wissel, K., Lenarz, T., Werner, D., Reuter, G., Kral, A. and Warnecke, A.:?Dissociated Neurons and Glial Cells Derived from Rat Inferior Colliculi After Digestion with Papain.,?PLoS ONE?8, e80490, 2013
大鼠 Rat, SD, 19-21 day 視交叉上核神經元 / Suprachiasmatic nucleus neurons Papain: 100 u/ml MEM Cao, R., Li, A., Cho, H., Lee, B. and Obrietan, K.:?Mammalian Target of Rapamycin Signaling Modulates Photic Entrainment of the Suprachiasmatic Circadian Clock.,?J Neurosci 30, 6302, 2010
大鼠 Rat, Fisher, 7-21 month 海馬神經元 / Hippocampal neurons Papain: 0.2% Hibernate A Chen, N., Newcomb, J., Garbuzova-Davis, S., Davis Sanberg, C., Sanberg, P. and Willing, A.:?Human Umbilical Cord Blood Cells Have Trophic Effects on Young and Aging Hippocampal Neurons in Vitro.,?Aging Dis 1, 173, 2010
大鼠 Rat, SD, 7 day 小腦顆粒神經元 / Cerebellar granule neurons PDS kit: per instructions PBS Tanaka, S., Shaikh, I., Chiocca, E. and Saeki, Y.:?The Gs-Linked Receptor GPR3 Inhibits the Proliferation of Cerebellar Granule Cells During Postnatal Development.,?PLoS ONE?4, e5922, 2009
大鼠 Rat, neonatal 星形膠質細胞 / Astrocytes Papain: 20 u/ml EBSS Shigetomi, E. and Khakh, B.:?Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes.,?J Vis Exp?26, 1142, 2009
大鼠 Rat, neonatal 海馬神經元 / Hippocampal neurons Papain: 10 u/ml EBSS Richler Esther, Chaumont Severine, Shigetomi Eiji, Sagasti Alvaro, Khakh Baljit S:?Tracking transmitter-gated P2X cation channel activation in vitro and in vivo,?Nat Methods?5, 87-93, 2008
大鼠 Rat, SD 海馬 / Hippocampal Trypsin: 0.1% Neurobasal Akanda, N., Tofighi, R., Brask, J., Tamm, C., Elinder, F. and Ceccatelli, S.:?Voltage-Dependent Anion Channels (VDAC) in the Plasma Membrane Play a Critical Role in Apoptosis in Differentiated Hippocampal Neurons but not in Neural Stem Cells.,?Cell Cycle 7, 3225, 2008
大鼠 Rat, neonatal 硬腦膜 / Dura mater Collagenase Type 1: 0.075% DMEM Peptan, I., Hong, L. and Evans, C.:?Multiple Differentiation Potentials of Neonatal Dura Mater-Derived Cells.,?Neurosurgery?60, 346, 2007
大鼠 Rat, E18 海馬神經元 / Hippocampal neurons Papain: 0.2% Hibernate Jekabsons MB, Nicholls DG:?Bioenergetic analysis of cerebellar granule neurons undergoing apoptosis by potassium/serum deprivation,?Cell Death Differ?13, 1595-610, 2006
大鼠 Rat, Wistar, 1-3 day 海馬神經元 / Hippocampal neurons Trypsin: 0.05% DMEM Velasco Myrian, Garci苔 Esperanza, Onetti Carlos G:?Glucose deprivation activates diversity of potassium channels in cultured rat hippocampal neurons,?Cell Mol Neurobiol 26, 307-19, 2006
大鼠 Rat, E19 海馬神經元 / Hippocampal neuron Papain: 10 u/ml MEM Khakh Baljit S, Fisher James A, Nashmi Raad, Bowser David N, Lester Henry A:?An angstrom scale interaction between plasma membrane ATP-gated P2X2 and alpha4beta2 nicotinic channels measured with fluorescence resonance energy transfer and total internal reflection fluorescence microscopy,?J Neurosci 25, 6911-20, 2005
大鼠 Rat, Wistar, 14 day 視覺皮層 / Visual cortical PDS kit: per instructions EBSS Mizoguchi Y, Kanematsu T, Hirata M, Nabekura J:?A rapid increase in the total number of cell surface functional GABAA receptors induced by brain-derived neurotrophic factor in rat visual cortex,?J Biol Chem 278, 44097, 2003
大鼠 Rat, Wistar, male 腦內皮 / Cerebral endothelial Collagenase Type 3: 0.2% MEM Floris S, Van den Born J, van der Pol SM, Dijkstra CD, De Vries HE:?Heparan sulfate proteoglycans modulate monocyte migration across cerebral endothelium,?J Neuropathol Exp Neurol 62, 780, 2003
大鼠 Rat, SD, E18 皮質細胞 / Cortical cells Papain: 20 u/ml;
Deoxyribonuclease I: .005%
EBSS Behar, T, Smith, S, Kennedy, R, Mckenzie, J, Maric, I and Barker, J:?GABA(B) receptors mediate motility signals for migrating embryonic cortical cells,?Cereb Cortex 11, 744-53, 2001
大鼠 Rat, wistar, 250-300 g 腦動脈平滑肌細胞 / Cerebral artery smooth muscle cells Papain: 1.5 mg/ml;
Collagenase Type 4: 1.5 mg/ml
Physiological Salt Solution Brzezinska AK, Gebremedhin D, Chilian WM, Kalyanaraman B, Elliott SJ.:?Peroxynitrite reversibly inhibits Ca(2+)-activated K(+) channels in rat cerebral artery smooth muscle cells,?Am J Physiol Heart Circ Physiol?278, H1883, 2000
大鼠 Rat, SD, E16 皮層神經元 / Cortical neurons PDS kit: with modifications Neurobasal Medium Varney MA, Cosford ND, Jachec C, Rao SP, Sacaan A, Lin FF, Bleicher L, Santori EM, Flor PJ, Allgeier H, Gasparini F, Kuhn R, Hess SD, Velicelebi G, Johnson EC:?SIB-1757 and SIB-1893: selective, noncompetitive antagonists of metabotropic glutamate receptor type 5,?J Pharmacol Exp Ther 290, 170, 1999
大鼠 Rat, wistar, neonatal 海馬細胞 / Hippocampal cells Papain: 1 mg/ml DMEM Boehm S, Betz H:?Somatostatin inhibits excitatory transmission at rat hippocampal synapses via presynaptic receptors,?J Neurosci 17, 4066, 1997
大鼠 Rat, SD, Fisher 海馬神經元 / Hippocampal neurons Papain: 0.2% HibernateA/B27 Brewer, G.J.:?Isolation and culture of adult rat hippocampal neurons of any age,?J Neurosci Methods 71, 143, 1997
大鼠 Rat, E18 海馬和皮層神經元 / Hippocampal and cortical neurons PDS kit: per instructions Neurobasal media Naeve GS, Ramakrishnan M, Kramer R, Hevroni D, Citri Y, Theill LE:?Neuritin: a gene induced by neural activity and neurotrophins that promotes neuritogenesis,?Proc Natl Acad Sci U S A 94, 2648-53, 1997
大鼠 Rat, SD, newborn 膠質細胞 / Glial Trypsin: 0.0625% MEM, sterile Pixley, S.K.:?The Olfactory Nerve Contains Two Populations of Glia,?In Vitro 5, 269, 1992
大鼠 Rat, newborn 星形膠質細胞 / Astrocytes Trypsin: 0.25% DMEM, HBSS Holzwarth, J., Glaum, S., and Miller, R.:?Activation of Endothelin Receptors by Sarafotoxin Regulates Ca2+ Homeostasis in Cerebellar Astrocytes,?Glia 5, 239, 1992
大鼠 Rat, 60-72 hours old 成纖維細胞 / Fibroblasts Trypsin: 0.2% HEPES buffered DMEM Acheson, A., Barker, P., Alderson, R., Miller, F., and Murphy, R.:?Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF,?Neuron 7, 265, 1991
大鼠 Rat, SD, adult 內皮 / Endothelial Collagenase Type 2: 0.5% Medium 199 Doron, D., Jacobowitz, D., Heldman, E., Feurerstein, G., Pollard, H., and Hallenbeck, J.:?Extracellular Matrix Permits the Expression of Von Willebrand's Factor, Uptake of Di-I-Acetylated Low Density Lipoprotein and Secretion of Prostacyclin in Cultures of Endothelial Cells from Rat Brain Microvessels,?In Vitro Cell Dev Biol 27A, 689, 1991
大鼠 Rat, SD, 5 day 海馬神經元 / Hippocampal neurons Trypsin: 0.2%;
Deoxyribonuclease I: 10 ug/ml
HBSS Mattson, M., and Kater, S.:?Development and Selective Neurodegeneration in Cell Cultures From Different Hippocampal Regions,?Brain Res 490, 110, 1989
大鼠 Rat, SD, female 胎鼠大腦 / Fetal rat brain Collagenase Type 4: 0.1% DMEM Matsuda, M.:?Serum Proteins Enhance Aggregate Formation of Dissociated Fetal Rat Brain Cells in an Aggregating Culture,?In Vitro Cell Dev Biol 24 (10), 1031, 1988
大鼠 Rat, albino, adult and newborn 大腦皮質 / Cerebral cortices Trypsin: 0.25% PBS Giulian, D. and Baker, T.J.:?Characterization of Ameboid Microglia Isolated From Developing Mammalian Brain,?J Neurosci 6, 2163, 1986
大鼠 Rat, Wistar, newborn 生發基質細胞 / Germinal matrix Trypsin: 0.25% HBSS Goldman, J.E., Geier, S.S., and Hirano, M.:?Differentiation of Astrocytes and Oligodendrocytes From Germinal Matrix Cells in Primary Culture,?J Neurosci 6, 52, 1986
大鼠 Rat, fetus, 18-20 day 海馬神經元 / Hippocampal neurons Trypsin: 0.25% HBSS, CMF Bartlett, W. and Banker, G.:?An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts,?J Neurosci 4, 1944, 1984
大鼠 Rat, fetus, 18 day 海馬 / Hippocampi Trypsin: 0.1% HBSS Rothman, S.:?Synaptic Release of Excitatory Amino Acid Neurotransmitter Mediates Anoxic Neuronal Death,?J Neurosci 4 (7), 1884, 1984
大鼠 Rat, SD, 19-20 days pregnant 神經 / Neural Trypsin: 0.25% DMEM Ahmed, Z., Walker, P., and Fellows, R.:?Properties of Neurons from Dissociated Fetal Rat Brain in Serum-Free Culture,?J Neurosci 3 (12), 2448, 1983
大鼠 Rat, SD, fetal 大腦皮層,下丘腦 / Cerebral cortex,Hypothalmus Deoxyribonuclease I: 0.001% HEPES Peterfreund, R. and Vale, W.:?High Molecular Weight Somatostatin Secretion by Cultured Rat Brain Cell,?Brain Res 239, 463, 1982
大鼠 Rat, Wistar-Kyoto, male, 100 - 200 g 內皮,腦 / Endothelial,Cerebral Collagenase Type 2: 0.05% HBSS Diglio, C.A., Grammas, P., Filiberto Giacomelli, M.S., and Wiener, J.:?Primary Culture of Rat Cerebral Microvascular Endothelial Cells,?Lab Invest 46, 554, 1982
大鼠 Rat, fetus 皮質 / Cortical Trypsin: 0.25% Puck's D1 (see reference) Swaiman, K., Neale, E., Fitzgerald, S., and Nelson, P.:?A Method for Large-scale Production of Mouse Brain Cortical Cultures,?Brain Res 255, 361, 1982
大鼠 Rat, Wistar, pregnant 膠質細胞 / Glial Trypsin: 0.05% Eagle's MEM/DMEM Abney, E., Bartlett, P., and Raff, M.:?Astrocytes, Ependymal Cells, and Oligodendrocytes Develop on Schedule in Dissociated Cell Cultures of Embryonic Rat Brain,?Dev Biol 83, 301, 1981
大鼠 Rat, SD, one month old 毛細血管內皮,周細胞 / Capillary endothelium,Pericytes Neutral Protease: 0.1% Medium 199 Bowman, P., Betz, A., Ar, D., Wolinsky, J., Penney, J., Shivers, R., and Goldstein, G.:?Primary Culture of Capillary Endothelium From Rat Brain,?In Vitro 17 (4), 353, 1981
大鼠 Rat, Wistar, male, 300-500 g 微血管,內皮 / Microvessels,Endothelial Collagenase Type 2: 0.75% Ringers-HEPES buffer Williams, S., Gillis, J., Matthews, M., Wagner, R., and Bitensky, M.:?Isolation and Characterization of Brain Endothelial Cells: Morphology and Enzyme Activity,?J Neurochem 35 (2), 374, 1980
大鼠 Rat, Wistar, adult, 170 g 內皮 / Endothelial Trypsin: 0.5% BSS Phillips, P., Kumar, P., Kumar, S., and Waghe, M.:?Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter,?J Anat 129, 261, 1979
大鼠 Rat, Holtzmann, 18 day 海馬神經元 / Hippocampal neurons Trypsin: 0.1% HBSS Banker, G., and Cowan, M.:?Rat Hippocampal Neurons in Dispersed Cell Culture,?Brain Res 126, 397, 1977
大鼠 Rat, SD, 2 days 松果體 / Pineal Trypsin: 0.25% DMEM, MEM Rowe, V., Neale, E., Avins, L., Guroff, G., and Schrier, B.:?Pineal gland cells in culture. Morphology, Biochemistry, Differentiation, and co-culture with sympathetic neurons,?Exp Cell Res?104, 345, 1977
貝類 Helisoma trivolvis 神經元,頰神經節 / Neurons,Buccal ganglia Trypsin: 0.2% Saline, Sterile Hadley, R.D., Bodnar, D.A., and Kater, S.B.:?Formation of Electrical Synapses Between Isolated, Cultured Helisoma Neurons Requires Mutual Neurite Elongation,?J Neurosci 5, 3145, 1985
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