貨號 產品 描述 選擇指南
RNT-SCI-10010500 抗 dsRNA 單克隆抗體 J2 小鼠,IgG2a,κ 輕鏈 IVT體外轉錄質控,金標準;文章最多
RNT-SCI-10040500 抗 dsRNA 單克隆抗體 J5 小鼠,IgG2b,κ 輕鏈 更適合IF實驗
RNT-SCI-10020500 抗 dsRNA 單克隆抗體 K1 小鼠,IgG2a,κ 輕鏈 更適合?Poly I:C 檢測
RNT-SCI-10050100 抗 dsRNA ,抗體套裝 J2、J5 和 K1 抗體的尺寸樣本 推薦用于 J2、J5 和 K1 抗體的比較測試
RNT-SCI-10030010 抗 dsRNA 單克隆抗體 K2 小鼠,IgM,κ 輕鏈 更適合ELISA
RNT-SCI-10080100 dsRNA 142 bp 合成 dsRNA 陽性對照

Fig.1:A)使用抗雙鏈RNA單克隆抗體J2在SARS-CoV感染的Vero細胞中檢測dsRNA的免疫熒光。 1:未感染的細胞。 標尺:20微米(根據[4]進行修改)。 B)使用抗雙鏈RNA單克隆抗體J2在SARS-Cov感染的Vero E6細胞的雙膜囊泡(DMVs)中進行免疫電子顯微鏡檢測(根據[5]進行修改)。 C)使用抗雙鏈RNA單克隆抗體J2在柯薩奇病毒感染的新生小鼠FFPE組織中檢測dsRNA。 1:小鼠心臟,2:褐色脂肪,3:胰腺,4:唾液腺,5:中樞神經系統神經節,6:未感染組織。



SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9,Cell??|??2023 Oct 26

Article Snippet

".. washed once with ice-cold PBS, followed by two washes with PBS at room temperature.. For combining immunostaining with HCR-based detection, we used?anti-J2 antibody?(Jena Bioscience,?RNT-SCI-10010200, 1:500) or anti-SND1 antibody (Proteintech, 60265-1- Ig, 1:500).. Incubation with the primary antibody was performed for 1 h at room temperature.Incubation with .."

Figure Legend
"... (B) Representative images of IF staining for dsRNA?(J2?antibody)?using?HCR?detection in SARS-CoV-2 infected"



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[2] Lukacs (1994) Detection of virus infection in plants and differentiation between coexisting viruses by monoclonal antibodies to double-stranded RNA. J. Virol. Methods47: 255.

[3] Lukacs (1997) Detection of sense:antisense duplexes by structure-specific anti-RNA antibodies. In: Antisense Technology. A Practical Approach, C. Lichtenstein and W. Nellen (eds), pp. 281-295. IRL Press, Oxford

[4] Weber et al. (2006) Double-Stranded RNA is produced by positive-strand RNA viruses and DNA viruses but not in detectable amounts by negative-strand RNA viruses. Journal of Virology 80(10): 5059.

[5] Knoops et al. (2008) SARS-Coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum. PLOS Biology 6(9): e226.

[6] Richardson et al. (2010) Use of antisera directed against dsRNA to detect viral infections in formalin-fixed paraffin-embedded tissue. Journal of Clinical Virology 49: 180.

[7] Karikó et al. (2011) Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA. Nucleic Acids Research 39(21): e142.



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