植物研究必備,細胞質膜H+-ATPase抗體

 

細胞質膜

 

植物細胞膜H+-ATPase,廣泛分布于植物細胞質膜上的ATP酶,由Hodges等人于1972年研究質膜微囊的ATPase的水解活力時發現。根據功能和位置不同,植物細胞內的H+-ATPase可分為F、P和V三大類型:F型ATPase主要分布在線粒體和葉綠體上;P型ATPase分布在質膜上;V型ATPase分布在液泡膜、內質網膜、溶酶體上。今天的主角是,P型H+-ATPase!(下文提及均為P型H+-ATPase)

 

質膜H+-ATP酶(P型H+-ATPase)是一個約100kDa的蛋白質家族,被認為是植物和真菌質膜的專屬蛋白。該蛋白被固定在生物膜內,形成電化學梯度,作為能量來源,對大多數代謝物的吸收和植物對環境的反應(如葉子的移動,氣孔開合,花粉管生長..)至關重要。

 

代謝物的吸收和植物對環境的反應

 

因為H+-ATPase極其重要,且廣泛分布在各類植物物種中,因此,絕大多數植物研究工作者的科學研究,幾乎都逃不開一支靠譜的,多應用,多文章的H+-ATPase抗體。

 

艾美捷科技作為專業的生命科學領域整體解決方案供應商,為您推薦超好用的胞質膜H+-ATPase抗體:

 

貨號 AS07 260
名稱 H+ATPase | Plasma membrane H+ATPase
質膜H+-ATP酶
應用類型 ?1 : 600-1 : 1000 (IF), 1 : 100 (IL), 1 : 1000-1 : 10 000 (WB)
驗證過的反應種屬 七葉樹,擬南芥,茶樹, 輪藻R.Br, 萊茵衣藻, 黃瓜, 南瓜, 大豆, 秋茄, 大麥, 黑麥草, 番茄, 蘋果, 地錢, 蒺藜苜蓿, 煙草, Zn超富集植物, 水稻, 矮牽牛, 蝴蝶蘭V3, 小立碗蘚, 云杉, 歐洲山楊, 鳳尾蕨, 蓖麻, 菠菜, 玉米, 蠶豆
不反應種屬 黑曲霉
形式 凍干粉;使用前,用50ul無菌去離子水溶解.
預期分子量 ?90- 95 kDa(擬南芥;具體根據不同種屬亞型,上下浮動)
說明書 點我下載
植物膜專題 Agrisera植物膜運輸系統抗體,幫您從外到內解開面紗

 

H+-ATPase實驗結果展示:

H+-ATPase實驗結果

WB:1: 5000

IF:1: 300

 

使用H+-ATPase抗體,發高分文章:

 

Wang?et al. (2020). Plant NLR Immune Receptor Tm-22 Activation Requires NB-ARC Domain-Mediated Self-Association of CC Domain. PLoS Pathog. 2020 Apr 27;16(4):e1008475. doi: 10.1371/journal.ppat.1008475.

Collins?et al. (2020). EPSIN1 Modulates the Plasma Membrane Abundance of FLAGELLIN SENSING2 for Effective Immune Responses . Plant Physiol. 2020 Feb 24. pii: pp.01172.2019. doi: 10.1104/pp.19.01172

Wang?et al. (2020). The Arabidopsis exocyst subunits EXO70B1 and EXO70B2 regulate FLS2 homeostasis at the plasma membrane. New Phytol. 2020 Mar 2. doi: 10.1111/nph.16515.

Kuang?et al. (2019). Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins. Front Plant Sci. 2019 May 15;10:641. doi: 10.3389/fpls.2019.00641. eCollection 2019.

Yuan?et al. (2019). Phospholipidase Dδ Negatively Regulates the Function of Resistance to Pseudomonas syringae pv. Maculicola 1 (RPM1). Front Plant Sci. 2019 Jan 18;9:1991. doi: 10.3389/fpls.2018.01991.

Zhang?et all. (2018). Root plasma membrane H+-ATPase is involved in low pH-inhibited nitrogen accumulation in tea plants (Camellia sinensis L.). Plant Growth Regul (2018) 86: 423.

Roth?et al. (2018). A rice Serine/Threonine receptor-like kinase regulates arbuscular mycorrhizal symbiosis at the peri-arbuscular membrane. Nat Commun. 2018 Nov 8;9(1):4677. doi: 10.1038/s41467-018-06865-z.

Wang?et al. (2018). Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity. Proc Natl Acad Sci U S A. 2018 Nov 16. pii: 201813058. doi: 10.1073/pnas.1813058115.

Pertl-Obermeyer?et al. (2018). Dissecting the subcellular membrane proteome reveals enrichment of H+ (co-)transporters and vesicle trafficking proteins in acidic zones of Chara internodal cells. PLoS One. 2018 Aug 29;13(8):e0201480. doi: 10.1371/journal.pone.0201480.

Zhang?et al. (2018). Maintenance of mesophyll potassium and regulation of plasma membrane H+-ATPase are associated with physiological responses of tea plants to drought and subsequent rehydration. The Crop Journal July 2018. (Camellia sinensis)

Seguel?et al. (2018). PROHIBITIN 3 forms complexes with ISOCHORISMATE SYNTHASE 1 to regulate stress-induced salicylic acid biosynthesis in Arabidopsis. Plant Physiol. Jan 2018. DOI:10.1104/pp.17.00941

Duan?et al. (2017). A Lipid-Anchored NAC Transcription Factor Is Translocated into the Nucleus and Activates Glyoxalase I Expression during Drought Stress. Plant Cell. 2017 Jul;29(7):1748-1772. doi: 10.1105/tpc.17.00044. (Nicotiana benthamiana)

Nagel?et al. (2017). Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017 Aug 7. pii: 201710866. doi: 10.1073/pnas.1710866114.

Aloui?et al. (2017). The plasma membrane proteome of Medicago truncatula roots as modified by arbuscular mycorrhizal symbiosis. Mycorrhiza. 2017 Jul 19. doi: 10.1007/s00572-017-0789-5.

Lomin?et al. (2017). Studies of cytokinin receptor–phosphotransmitter interaction provide evidences for the initiation of cytokinin signalling in the endoplasmic reticulum. Functional Plant Biology, CSIRO Publications. (Nicotiana benthamiana, western blot)

Kovaleva?et al. (2017). Regulation of Petunia Pollen Tube Growth by Phytohormones: Identification of Their Potential Targets. DOI:10.17265/2161-6256/2016.04.004. (immunolocalization)

Liao?et al. (2017). Arabidopsis E3 ubiquitin ligase PLANT U-BOX13 (PUB13) regulates chitin receptor LYSIN MOTIF RECEPTOR KINASE5 (LYK5) protein abundance. New Phytol. 2017 Feb 14. doi: 10.1111/nph.14472.

LaMontagne?et al. (2016). Isolation of Microsomal Membrane Proteins from Arabidopsis thaliana. Curr. Protoc. Plant Biol. 1:217-234. doi: 10.1002/cppb.20020.

Heard?et al. (2015). Identification of Regulatory and Cargo Proteins of Endosomal and Secretory Pathways in Arabidopsis thaliana by Proteomic Dissection. Mol Cell Proteomics. 2015 Jul;14(7):1796-813. doi: 10.1074/mcp.M115.050286. Epub 2015 Apr 21.

 

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