墻裂推薦直接法:8-羥基脫氧鳥苷(8-OHdG)定量試劑盒

8-羥基脫氧鳥苷

 

活性氧(ROS)自由基可以直接攻擊生物大分子DNA誘發DNA氧化損傷。在各種氧化損傷中,鳥苷是DNA核堿基中最易被氧化的堿基,羥基自由基和超氧陰離子會直接攻擊DNA分子中鳥嘌呤堿基的第8位碳原子,從而生成氧化性加合物8-OhdG(8-羥基脫氧鳥苷)。

 

8-OhdG(8-羥基脫氧鳥苷)

 

8-OHdG現已被廣泛地應用在實驗研究和臨床診斷中,參與氧化應激與病變的指標檢測與機理分析,對心血管疾病、糖尿病、神經退行性疾?。ㄅ两鹕?,阿爾茨海默氏癥)、以及癌癥的臨床診療都具有極大的應用價值。目前市場上主流使用的是競爭ELISA方法,因為它們不夠精確,也不便于應用于細胞/組織的8-OHdG檢測,更不能直接檢測從細胞或組織分離的完整DNA。

 

為此,艾美捷科技作為專業的生命科學領域解決方案供應商,為您推薦全球最新的8-OHdG直接定量試劑盒。其檢測準確度與HPLC或LC-MS分析高度相關和接近。

 

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產品名稱及描述 貨號 產品說明
EpiQuik 8-OHdG DNA Damage Quantification Direct Kit (Colorimetric) P-6003 詳情

*8-OHdG試劑盒可直接檢測提取好的DNA樣品

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8-羥基脫氧鳥苷試劑盒原理

DNA包被特殊處理帶有高DNA親和性的微孔板上,8-OHdG通過捕獲抗體和檢測抗體來檢測.通過酶標儀可以檢測結果.大量得8-OHdG與光密度值成比例.

 

8-OHdG試劑盒實驗流程

<8-OHdG試劑盒實驗流程,標準曲線,以及與液相質譜分析對比>

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8-OHdG試劑盒優勢:

1、比色實驗簡單易用,方便快速,整個試驗完成需要3小時45分鐘;

2、高靈敏度,檢測8-OHdG極限低至2pg;

3、高特異性,只能檢測8-OHdG,不能檢測8-OHdG類似物;

4、利用完整DNA直接檢測8-OHdG,能消除高分子量化合物的影響,例如:糖類和蛋白質等能競爭8-OHdG;

5、檢測結果精確,近似接近于HPLC和LC-MS;

6、高度方便直接使用來自細胞,組織中的DNA,不需要DNA消化或者水解;

7、包含通用的陽性對照和陰性對照,能適用于定量任何種系8-OHdG;

8、本試劑盒實驗結果更精確,靈敏,可靠,可靠并能顯著降低背景信號.

 

《8-OHdG試劑盒已發表文章》

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Esplugas R?et. al.?(February 2018).?Renal and hepatic effects following neonatal exposure to low doses of Bisphenol-A and <sup>137</sup>Cs.?Food Chem Toxicol.?114:270-277.

Ceylan D?et. al.?(December 2017).?DNA redox modulations and global DNA methylation in Bipolar Disorder: Effects of sex, smoking and illness state?Psychiatry Research.

Haque S?et. al.?(November 2017).?Monocyte-derived exosomes upon exposure to cigarette smoke condensate alter their characteristics and show protective effect against cytotoxicity and HIV-1 replication.?Sci Rep.?7(1):16120.

Tatsuki Kunoh?et. al.?(October 2017).?Green Synthesis of Gold Nanoparticles Coupled with Nucleic Acid Oxidation?ACS Sustainable Chemistry & Engineering.

Zhenqiang You?et. al.?(September 2017).?Modulatory Effect of Fermented Papaya Extracts on Mammary Gland Hyperplasia Induced by Estrogen and Progestin in Female Rats?Oxidative Medicine and Cellular Longevity.?2017

Hara K?et. al.?(September 2017).?Scavenging of reactive oxygen species by astaxanthin inhibits epithelial-mesenchymal transition in high glucose-stimulated mesothelial cells.?PLoS One.?12(9):e0184332.

Afshari P?et. al.?(September 2017).?Reduced Slc1a1 expression is associated with neuroinflammation and impaired sensorimotor gating and cognitive performance in mice: Implications for schizophrenia.?PLoS One.?12(9):e0183854.

  1. Tvrda?et. al.(July 2017).?Antioxidant effects of lycopene on bovine sperm survival and oxidative profile following cryopreservationVeterinarni Medicina.?62(8):429–436.

Bellés M?et. al.?(March 2017).?Environmental exposure to low-doses of ionizing radiation. Effects on early nephrotoxicity in mice.?Environ Res.?156:291-296.

Noguera JC?et. al.?(March 2017).?Interacting effects of early dietary conditions and reproductive effort on the oxidative costs of reproduction.?PeerJ.?5:e3094.

Ng CT?et. al.?(February 2017).?Zinc oxide nanoparticles exhibit cytotoxicity and genotoxicity through oxidative stress responses in human lung fibroblasts and <i>Drosophila melanogaster</i>.?Int J Nanomedicine.?12:1621-1637.

Tasaki E?et. al.?(January 2017).?An Efficient Antioxidant System in a Long-Lived Termite Queen.?PLoS One.?12(1):e0167412.

Daniel Nettle?et. al.?(December 2016).?Early-life adversity accelerates cellular ageing and affects adult inflammation: Experimental evidence from the European starling?Scientific Reports.?Dec. 2016

Szmidt M?et. al.?(July 2016).?Toxicity of different forms of graphene in a chicken embryo model.?Environ Sci Pollut Res Int.

Adamczyk J?et. al.?(June 2016).?Copy number variations of genes involved in stress responses reflect the redox state and DNA damage in brewing yeasts.?Cell Stress Chaperones.

Yost AD?et. al.?(October 2015).?Atmospheric Nonthermal Plasma-Treated PBS Inactivates Escherichia coli by Oxidative DNA Damage.?PLoS One.?10(10):e0139903.

Deregowska A?et. al.?(October 2015).?Genome-wide array-CGH analysis reveals YRF1 gene copy number variation that modulates genetic stability in distillery yeasts.?Oncotarget.?6(31):30650-63.

Philbrook NA?et. al.?(October 2015).?Benzoquinone toxicity is not prevented by sulforaphane in CD-1 mouse fetal liver cells.?J Appl Toxicol.

Shimizu R?et. al.?(April 2015).?Cholangiocyte senescence caused by lysophosphatidylcholine as a potential implication in carcinogenesis.?J Hepatobiliary Pancreat Sci.

Nocchi L?et. al.?(June 2014).?Induction of oxidative stress causes functional alterations in mouse urothelium via a TRPM8-mediated mechanism: implications for aging.?Aging Cell.?13(3):540-50.

Di Nunzio M?et. al.?(November 2013).?Counteraction of oxidative damage by pomegranate juice: influence of the cultivar.?J Sci Food Agric.?93(14):3565-73.

Sobinoff AP?et. al.?(September 2013).?Scrambled and fried: cigarette smoke exposure causes antral follicle destruction and oocyte dysfunction through oxidative stress.?Toxicol Appl Pharmacol.?271(2):156-67.

 

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